Natural plants are prospective sources of the new antimicrobial drug. This study found some natural materials from plants that have antimicrobial effect. The antibacterial activity and the minimum bactericidal concentration of methanol and ethanol extracted from 18 plants against Escherichia coli, Bacillus subtilis, Staphylococcus aureus studied in this paper. As analyzed its terms and bacteriostatic conditions, we found that Cyperus rotundus Linn, Camptotheca acuminate Decne, EucaLyptus spp, Lonicera japonica Thunb showed good antibacterial activity. The results could provide a reference for extracting antibacterial components from these plants.