The purpose of present study was to develop fenugreek as a release rate retardant excipient source. The matrix type sustained release tablets of Lamivudine were formulated using fenugreek polymer, gum tragacanth and gaur gum by wet granulation method. The three factor two level Box-Behnken design was selected for formulation development. The tablet was evaluated for hardness, friability test, wet variation test and drug content test. Also, effect of hardness of tablet, binder concentration (fenugreek polymer, gum tragacanth and gaur gum) and polymer concentration (HPMC k 100M) on the time required to release 35% of drug (t35) and percent release of drug was studied. Optimized batch of fenugreek polymer was predicted by software (Design Expert). The optimized batch (SSBF-OB) has given t35 up to 8.9 hrs and 97.09% drug release in a sustained way up to 24hrs. The comparative release potential of all the polymers was studied. In-vitro release data demonstrated that tablet with fenugreek polymer were successfully sustained the release of drug (97.09) up to 24 hrs as compare to the tablet with gum tragacanth (77.63%) and gaur gum (72.96%). LMV formulation by DOE showed that fenugreek-based polymer has a potential to act as release rate retardant excipients.