Bulbil of Dioscorea opposita is riched with nutrients, having extremely value of pharmacology research. In this study orthogonal test was used to choose the optimum conditions of polysaccharides extraction prepared by the ultrasonic-microwave from bulbil, including the different part of the bulbil, different microwave power, different solid-liquid ratio and different extract time. The results showed that the optimal extraction parameters were microwave power 500 W, extract time 30 min and solid-liquid ratio 1:25 (g/mL). Our experiment also showed that the yield rate of polysaccharide was 19.23%, which indicated that there was a good fit between the predicted value and the experimental value. These findings suggest that ultrasonic-microwave assisted extraction could greatly improve the extraction rate of the polysaccharides from bulbil.