Beta vulgaris is widely distributed in tropical and sub-tropical regions of Asia and Africa. Root and leave are edible. The root contains some pigments known as betalains. This plant is rich in vitamin C, folic acid, flavonoids and carotenoids. Beta vulgaris is used traditionally as hepatoprotective, hypotensive, hypoglycemic, vasodilating and anticancer. In this study, the GC-MS analysis revealed the following major constituents: (i) 9,12-octadecadienoic acid methyl ester (34.10%); hexadecanoic acid methyl ester (19.05%) (iii) 9-octadecenoic acid methyl ester (17.57%) and (iv) methyl stearate (8.68%). The oil was screened for antimicrobial activity. It showed significant activity against Bacillus subtilis and moderate anticandidal activity. it exhibited partial activity against other test organisms.