Phytochemical screening of Leptadenia pyrotechnica leaves revealed the presence of alkaloids, steroids, flavonoids, carbohydrates, tannins and saponins, while phytochemical screening of Acacia polyacantha stem revealed the presence of alkaloids, steroids, flavonoids, carbohydrates, and saponins. Tannins and glycosides were not detected. In the antimicrobial assay the n-butanol fraction of Acacia polyacantha stem showed significant activity against Bacillus subtilis and Pseudomonas aeruginosa, while the ethanol extract exhibited significant activity against Pseudomonas aeruginosa and Staphylococcus

aureus. The ethyl acetate fraction showed significant activity against Pseudomonas aeruginosa. All fractions of leaves

exhibited significant activity against Pseudomonas aeruginosa.

Keywords: Acacia polyacantha, Leptadenia pyrotechnica, phytochemical screening, antimicrobial activity