The purpose of this work was to formulate antiseptic ethanol 64% v/v gel. A number of trials were done in order to prepare a good hygienic gel using triethanolamine as gelling enhancer and glycerin as emollient. Different formulae were prepared using different percentages of carbomer. The formula (F 5) showed acceptable physical properties with good ability to spread on hands. The selected formula was subjected to microbiological evaluation. Results of microbiological evaluation proved that the gel formula F5 could decrease the microbiological count on hands.