Diplazium esculentum or “Pako” is an edible fern popularly used in homemade salads in the Philippines and the other form is used as an ornamental plant. According to the findings of Chawla et al. (2015) D. esculentum is a potential analgesic drug in treating pain and the active constituents inferred to be responsible for the effect were flavonoids and sterols. The present study was done to evaluate the analgesic property of the semi-purified flavonoids of D. esculentum ethanolic extract. Phytochemical analysis shown the presence of saponin glycosides, flavonoids, proteins and fixed oils. The evaluation used acetic acid induced writhing method in five groups of swiss albino mice given the dose of (25, 100, 300 and 500 mg/kg) of the plant extract compared to the positive control (Aspirin). The study used One Way Anova and Tukey’s Multiple Comparison test in the analysis of the data. The computed F which is 153.7977528 is greater than the F-critical which is 2.866081402 therefore there is a significant difference between the groups. The comparison used 95% level of confidence with a P value of 0.1209. These data may indicate that the semi-purified flavonoids of D. esculentum has showed analgesic effect as the dose increases.