Methods: The method was preferred with various columns like C18 column, koromasil, Hypersil BDS. Among them C18 was found to be ideal as it has good peak shape 1ml/min flow rate and a proper mobile phase was developed to obtain ideal and sharp peaks with optimum resolution.

Results: The system suitability parameters were evaluated from standard chromatogram and compared with sample chromatogram of aspirin, clopidogrel, and rosuvastatin. Also there peak areas and retention time were observed to obtain an ideal peak. Several mobile trials were performed and at 14th trial a proper chromatogram was generated with ideal peak obtained for all the three dugs similar to that of standard.

Conclusion: The retention times for Aspirin, Clopidogrel, and Rosuvastatin were 8.933, 4.953, and 6.783 respectively and pure peaks were obtained. Therefore a new method was developed for estimation of aspirin, clopidogrel and rosuvastatin us in RP-HPLC method.