In the present study, dry seeds of C. decurrens were treated with different concentration of colchicine(0.5,1 and 2%), to increase the synthesis and accumulation of secondary metabolite as well as changing the genetic activity which may lead to increased chance of obtaining high yielding plant variety. In the study highest doses of colchicine exhibited enhanced shoot and root length, no. of leaves and node. In contrast to that fresh weight was significant decreased in 2% colchicine as against in control. Chlorophyll content (chl a, b and total chl) showed great variability as well as uneven distribution of chlorophyll pigment irrespective of colchicine doses was found. In quantitative HPTLC analysis of seconday metabolite (mangiferin, scopoletin and quercetin) in ethanolic micro extracts all doses of colchicines was found to effective increasing the active phytochemical, quantity of scopoletin was found highest in 1% than control.