Luteolin is a plant flavonoid and pharmacologically active agent that has been isolated from several plant species. In the present study, fractionation using methanol, petroleum ether, and ethyl acetate was performed to extract luteolin from the nutshell waste, Arachis hypogea. Its antioxidant activity was then examined with DPPH radical scavenger method. Our results show that luteolin obtained from the nutshell waste has strong antioxidant activity (IC₅₀ = 8.772 ppm). Thus it would be useful to agro-solid waste management as well as pharmaceutical purposes.