The ink sac of Loligo duvauceli, generated as a byproduct during processing, has low market value and has the potential to cause environmental pollution. Antioxidant and antibacterial activities of crude and partially purified form of Loligo duvauceli ink has been largely studied. The in vitro free radical scavenging activity of crude and partially purified form of Loligo duvauceli ink was conducted by using DPPH and Nitric oxide assay. The percentage of inhibition was calculated. The IC 50 value was calculated by plotting a graph, the percentage of inhibition against the concentration. From the IC 50 value the partially purified form shows higher free radical scavenging activity compared to the crude form of Loligo duvauceli ink.