Objective: The objective of our study is to clinically assess alterations in various serum paramaters (Magnesium, Bilirubin, C - reactive protein) in patients with migraine and compared with controls. Methods: A prospective study was conducted in Pushpagiri Medical College Hospital comprised of 50 migraine patients and equal number of healthy controls. Migraine Patients with or without aura are selected. Serum parameters such as magnesium, bilirubin and C- reactive protein is measured in patients who are willing to participate. These parameters are then quantitatively estimated using semi auto analyser. The results were analysed using t test and Chi square statistical analysis. Results: There were 50 migraine patients with a mean age group of 32.5 ± 5 years and 50 healthy individuals with a mean age group of 30.46 ± 5 years. The serum magnesium levels were found to be low in patients with migraine.It was also found that patients with migraine had a decreased levels of bilirubin and an increase in CRP. This shows that there is significant relation with the serum parameters in patients with migraine Conclusion: It is clear from our study that the serum parameters like CRP, Magnesium and bilirubin have specific role in the genesis of migraine and these parameters can be used as biomarker for diagnosis of migraine and also magnesium supplements can be used for the treatment of migraine.