Anoxia damage is major cause of morbidity and mortality in humans. In anoxia, oxygen supply decreased relative to metabolic demand. The response of Drosophila flies to many drugs that act within the central nervous system is similar to the effect observed in mammalian tissue. So, here we evaluate pharmacological potential of acamprosate in anoxia-reoxygenation (AR) injury in drosophila melanogaster. Anoxia reoxygenation injury was induced by introducing the pure nitrogen to the vial containing flies then flies were placed in room air for reoxygenation. Acamprosate shows survival advantage against AR injury. Acamprosate was observed to be protective against mortality due to anoxia reoxygenation injury at three concentrations 20, 100 and 200μg/ml. It was observed from our results that acamprosate increased the level of endogenous antioxidants (e.g. superoxide dismutase and catalase) and decrease the lipid peroxidation and level of nitric oxide.